Agriculture and Food SciencesBiotechnologies and Microorganisms
Expression (in E. coli), purification and biochemical/biophysical characterization of recombinant hydrolases (e.g. chitinase, β-1,3-glucanase); includes identification of activity-modulating ligands and testing of enzyme effects on selected phytopathogens.
Equipment: E. coli expression system, FPLC, spectrophotometer, protein biophysical characterization